Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus
Identifieur interne : 004150 ( Main/Exploration ); précédent : 004149; suivant : 004151Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus
Auteurs : J. W. Wang [République populaire de Chine] ; Y. P. Qi [République populaire de Chine] ; Y. X. Huang [République populaire de Chine] ; S. D. Li [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 1995-12-01.
English descriptors
- Teeft :
- Acetic acid, Amino, Amino acid, Amino acid residue, Amino acid residues, Amino acids, Autographa, Autographa californica, Baculovirus, Baculovirus gene, Baculovirus vector, Bombyx mori, Cagt motif, Californica, Cgtcg motif, Complete sequence, Core recognition sequence, Early gene, Early genes, Early promoter, Functional mapping, Gata, Gata motif, Gata motifs, Gene, Gene expression, Genome, Insect cells, Late phases, Late promoter sequence ttaag, Late promoters, Leucania seperata, Leucine, Leucine structure, Leucine zipper, Lsnpv, Lsnpv genome, Mini, Mini cistron, Molecular biology, Nucleotide, Nucleotide sequence, Other elements, Polyhedrosis, Polyhedrosis virus, Promoter, Prosis software, Putative protein, Random sequence, Recombinant plasmids, Regular leucine structures, Regulatory elements, Restriction sites, Sali, Sali fragment, Sali sites, Secondary structure, Sequencing, Small peptide, Tata, Tata boxes, Termination codon, Transcription, Tryptophane operon, Virol, Virology, Wuhan university.
Abstract
Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.
Url:
DOI: 10.1007/BF01323248
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus</title><author><name sortKey="Wang, J W" sort="Wang, J W" uniqKey="Wang J" first="J. W." last="Wang">J. W. Wang</name></author><author><name sortKey="Qi, Y P" sort="Qi, Y P" uniqKey="Qi Y" first="Y. P." last="Qi">Y. P. Qi</name></author><author><name sortKey="Huang, Y X" sort="Huang, Y X" uniqKey="Huang Y" first="Y. X." last="Huang">Y. X. Huang</name></author><author><name sortKey="Li, S D" sort="Li, S D" uniqKey="Li S" first="S. D." last="Li">S. D. Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4F3D6657EE30E88125C2F1783FDFCF1344A45559</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF01323248</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-KZ85176D-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C10</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C10</idno><idno type="wicri:Area/Istex/Curation">000C10</idno><idno type="wicri:Area/Istex/Checkpoint">001841</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001841</idno><idno type="wicri:doubleKey">0304-8608:1995:Wang J:nucleotide:sequence:of</idno><idno type="wicri:Area/Main/Merge">004211</idno><idno type="wicri:Area/Main/Curation">004150</idno><idno type="wicri:Area/Main/Exploration">004150</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus</title><author><name sortKey="Wang, J W" sort="Wang, J W" uniqKey="Wang J" first="J. W." last="Wang">J. W. Wang</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Wuhan University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Qi, Y P" sort="Qi, Y P" uniqKey="Qi Y" first="Y. P." last="Qi">Y. P. Qi</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Wuhan University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Huang, Y X" sort="Huang, Y X" uniqKey="Huang Y" first="Y. X." last="Huang">Y. X. 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Li</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Wuhan University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1995-12-01">1995-12-01</date><biblScope unit="volume">140</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="2283">2283</biblScope><biblScope unit="page" to="2291">2291</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetic acid</term><term>Amino</term><term>Amino acid</term><term>Amino acid residue</term><term>Amino acid residues</term><term>Amino acids</term><term>Autographa</term><term>Autographa californica</term><term>Baculovirus</term><term>Baculovirus gene</term><term>Baculovirus vector</term><term>Bombyx mori</term><term>Cagt motif</term><term>Californica</term><term>Cgtcg motif</term><term>Complete sequence</term><term>Core recognition sequence</term><term>Early gene</term><term>Early genes</term><term>Early promoter</term><term>Functional mapping</term><term>Gata</term><term>Gata motif</term><term>Gata motifs</term><term>Gene</term><term>Gene expression</term><term>Genome</term><term>Insect cells</term><term>Late phases</term><term>Late promoter sequence ttaag</term><term>Late promoters</term><term>Leucania seperata</term><term>Leucine</term><term>Leucine structure</term><term>Leucine zipper</term><term>Lsnpv</term><term>Lsnpv genome</term><term>Mini</term><term>Mini cistron</term><term>Molecular biology</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Other elements</term><term>Polyhedrosis</term><term>Polyhedrosis virus</term><term>Promoter</term><term>Prosis software</term><term>Putative protein</term><term>Random sequence</term><term>Recombinant plasmids</term><term>Regular leucine structures</term><term>Regulatory elements</term><term>Restriction sites</term><term>Sali</term><term>Sali fragment</term><term>Sali sites</term><term>Secondary structure</term><term>Sequencing</term><term>Small peptide</term><term>Tata</term><term>Tata boxes</term><term>Termination codon</term><term>Transcription</term><term>Tryptophane operon</term><term>Virol</term><term>Virology</term><term>Wuhan university</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Wang, J W" sort="Wang, J W" uniqKey="Wang J" first="J. W." last="Wang">J. W. Wang</name></region><name sortKey="Huang, Y X" sort="Huang, Y X" uniqKey="Huang Y" first="Y. X." last="Huang">Y. X. Huang</name><name sortKey="Li, S D" sort="Li, S D" uniqKey="Li S" first="S. D." last="Li">S. D. Li</name><name sortKey="Qi, Y P" sort="Qi, Y P" uniqKey="Qi Y" first="Y. P." last="Qi">Y. P. Qi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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